Download Lipid analysis and lipidomics : new techniques and by Magdi M Mossoba PDF

By Magdi M Mossoba

ISBN-10: 1893997855

ISBN-13: 9781893997851

Content material: strategy and functions in lipid research / NIls Hinrichsen and Hans Steinhart -- an outline of contemporary mass spectrometry tools within the toolbox of lipid chemists and biochemists / R. Moreau -- international mobile lipidome analyses by way of shotgun lipidomics utilizing multidimensional mass spectrometry / X. Han and R.W. Gross -- LC/MS and chiral separation / A. Kuksis and Y. Itabashi -- LC/MS and lipid oxidation / A. Kuksis and O. Sjovall -- Structural research of unsaturated fatty acid methyl ester isomers with acetonitrile covalent adduct chemical ionization (CACI) / J.T. Brenna -- contemporary advances in silver-ion HPLC using acetonitrile in hexane as solvent: temperature results on lipid elution orders/resolution / R.O. Adlof -- research of trans-18:1 fatty acids via silver ion HPLC / P. Delmonte, J.K.G. Kramer, and M.P. Yurawecz -- High-performance size-exclusion chromatography for lipid research in natural media / G. Márquez-Ruiz and M.C. Dobarganes -- Lipid separations utilizing packed-column supercritical fluid chromatography / D.G. Hayes --TLC-FID with particular connection with marine lipids and different high-molecular-weight natural compounds / R.G. Ackman and A. Timmins -- speedy GC for mobile status research of micro organism / J.S. patron. Use of mobile fatty acids to spot food-borne pathogens by means of infrared spectroscopy and capillary gasoline chromatography / M.M. Mossoba and S.F. Al-Khaldi -- program of FT-NIR for fast choice of the trans fatty acid composition in fat and oils / H. Azizian and J.K.G. Kramer -- Infrared spectroscopy and partial least sq. calibration within the simultaneous quantification of remoted trans and conjugated linoleic acids / A.A. Christy -- research of protein-lipid interactions by means of vibrational spectroscopy / G. Meng, N.K. Howell, and E.C.Y. Li-Chan -- fats replacers: an summary / W.E. Artz, S.M. Mahungu, and S.L. Hansen -- Phospholipids: buildings and physicochemical actions / M.C. Erickson -- Waxes and sterols: buildings and chemistry / E.J. Parish and A.D. Bell

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Example text

ESI, APCI, and APPI are all considered to be “atmospheric pressure ionization methods” (API) because the ionization occurs at atmospheric pressure. Several LC-MS manufacturers sell easily interchangeable ionization chambers that allow the use of ESI, APCI, and APPI (each in either the positive or negative ion mode) with the same instrument by attaching the appropriate spray chamber. The final soft ionization method that will be discussed is MALDI-MS. MALDI-MS requires a specialized instrument and because it involves mixing the analyte with a matrix and exposing the mixture to a laser, MALDI is not easily interfaced to an HPLC.

We would like to point out that the abundant PE pseudomolecular ions in the mass spectrum acquired in the negative-ion mode after addition of LiOH largely reflect the higher mass content of PE molecular species compared with individual anionic lipids in the lipid extract because all anionic phospholipid molecular species including PE species possess similar ionization efficiencies under the mildly basic experimental conditions. Therefore, one must consider whether anionic lipids in the extract could overlap with PE molecular species and thus affect their identification and quantitation.

Weakly anionic lipids) can be analyzed by negative-ion ESI-MS. Most of the other lipid classes Copyright (c) 2006 by AOCS Press Chapter03 2/25/06 3:06 PM 56 Page 56 X. W. Gross Fig. 2. Schematic diagram of shotgun lipidomics based on intrasource separation directly from a crude extract of a biological sample. , the solution used in the last step) in positive-ion ESI-MS because lipids in the first and second categories are now anionic under these conditions. Through this approach, a comprehensive series of mass spectra with respect to each of the aforementioned conditions can be obtained for each category of lipids.

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Lipid analysis and lipidomics : new techniques and applications by Magdi M Mossoba

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