By W. W. Christie
This is often the 3rd quantity of an occasional sequence of books facing facets of lipid methodology. The individuals speak about and study positional isomers of glycerolipids, long-chain acyl-coenzyme A esters, 31P nuclear magnetic resonance profiling of phospholipids and a few very important references in lipid method.
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Extra info for Advances in Lipid Methodology, Volume 3
The chiral YMC-Pack A-K03 column is able to separate also the reverse isomers ofrac-1,2-diacylglycerols of uneven fatty chain length (Y. ltabashi and A. Kuksis, 1990. Unpublished results). 3· 34 sn-2,3- 6 7 1 10 15 20 25 30 35 min. sn-1,2- ENANTIOMERIC DG OF CM·TG 34 sn-2,3- (ESTER-FED) sn-1,2· 34 sn-2,3- 10 15 20 25 30 Fig. 8. Initial resolution and rechromatography by chiral-phase HPLC of sn-1,2- and sn-2,3-diacylglycerol moieties of chylomicron triacylglycerols as the 3,5-DNPU derivatives [11 OJ.
The recent development of more robust 32 ANALYSIS OF POSITIONAL ISOMERS OF GLYCEROLIPIDS methods of stereospecific analysis of natural triacylglycerols promises rapid advances in a number of areas of application. Thus far, the diastereomer resolution of diacylglycerols has helped to characterize the lipolysis products of various lipases using synthetic achiral triacylglycerols and to make novel observations about the specificity of these enzymes [82,85,86]. Santinelli et al.  have used this method to demonstrate marked asymmetry between sn-1- and sn-3-positions of individual triacylglycerol species, although the stereospecific analysis of the total triacylglycerol mixture had indicated symmetry.
Am. Oil Chem. , 67, 487-494 (1990). ,J. Am. Oil Chem. , 68, 391-400 (1991 ). Yang,L-Y. ,J. , 32, 1173-1186 (1991). , Kuksis,A. J. , 31, 137-147 (1990). , Kuksis,A. ,J. , 36, 1046-1057 (1995). J. , J. , 36, 125-136 (1995). , Lorbeer,E. , Org. , 29, 253-259 (1994). Zollner,F. ,J. , 30, 432-437 (1995). Chapter2 31P NUCLEAR MAGNETIC RESONANCE PROFILING OF PHOSPHOLIPIDS Thomas Glonek1 and Thomas E. Merchant2 1MR Laboratory, Midwestern University, Chicago, Illinois 60615; 2Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Advances in Lipid Methodology, Volume 3 by W. W. Christie